Influence of organic solvents on papain kinetics.
نویسندگان
چکیده
Papain activity was studied in water-organic solvent mixtures using the fluorogenic substrate Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. The increase of organic solvent (MeOH, EtOH, iPrOH, TFE, MeCN, (MeO)2Et and DMF) concentration in the mixture caused a substantial decrease the initial rate of papain-catalyzed hydrolysis. Moreover, the number of papain active sites decreased with the increase of DMF and MeOH concentration.
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ورودعنوان ژورنال:
- Acta biochimica Polonica
دوره 48 4 شماره
صفحات -
تاریخ انتشار 2001